Microbiology of frozen orange concentrate. IV. Further studies of Enterococci in frozen orange concentrate.

the freeze-drying process. Continued viability of rehydrated cells during incubation at 37 C for 3 hours was dependent on the composition of the suspending medium. The number of viable organisms decreased greatly when suspended and incubated in water. Several salts were effective in maintaining higher viable cell counts. Maximum recovery of viable cells of one dried preparation rehydrated in malate solution was obtained in 0.05 M malate at pH 6.0, and at a temperature of 30C.